Cathie Wood-led Ark Investment Management on Monday bought 731,775 shares, estimated to be worth about $41.76 million, in Twitter Inc (NYSE:TWTR) — a day after The Bill and Melinda Gates Foundation Trust sold its entire stake in the social media company.

Shares of Twitter closed 4.81% higher at $57.06 on Monday. The stock has a 52-week high of $80.75 and a low of $28.23.  

The investment firm made the trades via two of its funds; the Ark Innovation ETF (NYSE:ARKK) and the ARK Next Generation Internet ETF (NYSE:ARKW).

The two ETFs together held over 10 million TWTR shares, worth about $549 million, as of Tuesday. No other Ark ETF holds shares of Twitter.

See also: How to Buy Twitter (TWTR) Stock

Twitter is steadily climbing up the Ark’s portfolio chart and is now ARKK's twenty-first largest investment among a total of 55 stocks and ARKW's fourth-largest investment among a total of 50 stocks. 

At the start of the month, it ranked as ARKK's thirty-fourth largest investment among a total of 58 stocks and ARKW's eighth-largest investment among a total of 53 stocks.

DraftKings shares closed 7.24% higher at $47.86 on Monday and were further up 1.34% in extended hours. 

Netflix shares closed 1% higher at $502.90 on Monday. 

See Also: Cathie Wood Buys $26M Disney Shares And Sells $13M In Netflix: What You Need To Know