ZIOPHARM
Oncology, Inc. ZIOP, a biopharmaceutical company focused on the
discovery and development of new cancer therapies, and Intrexon Corporation
XON, a leader in synthetic biology, today announced the presentation of
a preclinical study demonstrating the potential for using Intrexon's regulated
gene expression system, the RheoSwitch Therapeutic System^® (RTS^®), in human
mesenchymal stem cells (hMSCs). The study, "Regulated Immunomodulators
Expression Using the RheoSwitch Therapeutic System^® (RTS^®) Platform in Human
Mesenchymal Stem Cells," will be presented at the 2013 AACR-NCI-EORTC
International Conference on Molecular Targets and Cancer Therapeutics, which
is taking place October 19-23, 2013 in Boston.
hMSCs possess an inherent ability to traffic to tumors and sites of
inflammation, making them an attractive possibility as a cellular vehicle
delivery system, while the RTS^® platform is a novel regulated gene expression
system which, when activated by an orally available activator ligand (AL),
veledimex, can be used to express one or more anti-cancer effectors. Together,
these technologies offer the potential to optimize the delivery, dose and
schedule of one or more immunotherapies to achieve maximum therapeutic
efficacy and tolerability.
"In the lab and clinic, the RTS^® gene switch has been effectively combined
with various methods of delivery, including dendritic cells, as well as viral
and DNA plasmid vectors, to optimize our regulated therapeutic gene expression
systems," said Jonathan Lewis, M.D., Ph.D., Chief Executive Officer of
ZIOPHARM. "Mesenchymal stem cells, which naturally seek out cancer cells,
offer an exciting new method for delivering gene systems to cancer
cells. Together, these technologies give us unprecedented control over the
delivery and expression of one or more anti-cancer effectors."
"The therapeutic use of hMSCs is a field of study with rapidly growing
importance in a number of disease areas," said Samuel Broder, M.D., Senior
Vice President of Intrexon's Health Sector and former Director of the National
Cancer Institute. "For a cancer-directed gene expression system that relies on
a cellular host, it may be the ideal delivery mechanism. This study brings to
life the possibility of a self-guided cellular factory that can express
multiple anti-cancer effectors, on demand, directly at the tumor site."
For the study, ZIOPHARM and Intrexon evaluated RTS^® regulated expression of
three immunomodulators, human IL-12, human IFNα, and a CTLA4 decoy, in single,
dual or triple combinations, from adenovirally transduced hMSC. In a single
immunomodulator system, sustained cell levels of IL-12 expression were
observed up to 53 days following transduction when maintained in the presence
of veledimex. Furthermore, cycling of in vitro exposure periods between
veledimex and excipient demonstrated the ability for on/off/on and off/on/off
kinetics of IL-12 expression by transduced hMSCs.
For the multi-effector systems, hIL-12 and hIFNα were found to be fully
biofunctional in their respective cell based functional assays: hIL-12
increased IFNγ secretion from NK92 cells and hIFNα enhanced STAT1 reporter
activity. Further, the CTLA4 decoy demonstrated ability to inhibit protein
interaction in a cross-competitive assay. Taken together, these in vitro
studies highlight the potential use of MSCs for tumor-targeted delivery of
single or multiple RTS^® regulated cancer immunotherapies. The use of these
novel regulated immunotherapeutic approaches could potentially be translated
into an effective clinical regimen for a variety of cancers.
The study was conducted jointly between ZIOPHARM and Intrexon Corporation as
part of an Exclusive Channel Collaboration to research, develop and
commercialize novel in vivo DNA- and cell-based oncology therapeutics using
different approaches, all regulated by Intrexon's proprietary RheoSwitch
Therapeutic System^® (RTS^®) platform.
A copy of this poster presentation, by Chan et. al (abstract # C234), can be
found on ZIOPHARM's website.
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