Market Overview

NuGEN Launches mRNA-Seq Library Preparation Kits With NuQuant®, a Novel Library Quantification Method to Accurately Measure Molar Concentrations Of Next-Generation Sequencing (NGS) Libraries in Seconds

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NuGEN Technologies, a leading provider of NGS library preparation
solutions, today announced the launch of new mRNA-Seq
library preparation kits integrated with NuQuant
library
quantification method. This propriety library quantification method
enables direct measurement of NGS library molar concentration, or the
number of sequenceable molecules in seconds. The NuQuant
method eliminates the need to serially dilute libraries and estimate
library size, making it more accurate than other library quantitation
methods such as qPCR or Bioanalyzer®. NGS libraries can be
quantified using NuQuant method on standard fluorometers such as Tecan®
microplate readers
and Qubit. Accuracy of library quantification is
crucial for new Illumina® sequencers such as the NovaSeq®,
which has the sequencing capacity to generate 10 billion reads in a
single run. Inaccurate library quantification can lead to variability in
the number of reads per sample resulting in inconclusive data and
expensive resequencing of underrepresented samples.

Universal
Plus mRNA-Seq with NuQuant
is a complete, streamlined library
preparation solution for mRNA sequencing with a broad dynamic input
range from 10 ng to 1 μg. In addition to NuQuant, the kit comes standard
with Unique Dual Index (UDI) adaptors for each reaction in the kit,
providing a simple solution to detect and address "index hopping" issues
with Illumina sequencers. Universal Plus mRNA-Seq with NuQuant also
integrates NuGEN's proprietary DimerFree technology
to efficiently eliminate adaptor dimers regardless of input and
AnyDeplete technology to provide a unique option to
eliminate unwanted abundant transcripts after library construction.
NuGEN's NGS library preparation kits are automation friendly, compatible
with a variety of liquid-handling robots including Tecan's
automation platforms
.

"Universal Plus mRNA-Seq with NuQuant is the next step in NuGEN's
efforts to simplify and shorten the NGS library preparation process for
Illumina sequencing," said NuGEN CEO, Nitin Sood. "As more and more
researchers begin to do NGS, and as sequencing becomes cheaper, the
development of novel technologies to decrease library prep time and
cost, while maintaining performance and quality is essential. With
NuQuant, NuGEN once again demonstrates that it is at the forefront of
this process."

Tecan® is a registered trademark of Tecan Group. Bioanalyzer® is a
registered trademark of Agilent Technologies. Illumina® and NovaSeq® are
registered trademarks of Illumina, Inc.

About NuGEN Technologies, Inc.

Based in San Carlos, California, NuGEN Technologies, Inc., provides
innovative NGS and genomic sample prep products for a broad range of
sample types including RNA and DNA from whole tissues, FFPE samples,
single cells and liquid biopsies. NuGEN's products offer rapid and
simple workflows designed to reduce time and cost per sample. NuGEN
products are used by 1000s of customers in basic research, applied
markets and molecular diagnostics. NuGEN products are manufactured under
stringent quality controls (ISO 13485). The Tecan Group recently
acquired NuGEN Technologies, Inc. to further expand Tecan's dedicated
solutions offering into the new market segment of next-generation
sequencing (NGS) reagents. To learn more about NuGEN visit www.nugen.com.

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